Thesis Dissertation Defense

Thesis Dissertation Defense

Hybridization and dehybridization of short oligonucleotides subject

Date

September 23, 2022 - 10:00am to 11:00am

Location

Howey Physics Building

Room

N110

Speaker

Affiliation

Georgia Institute of Technology
Abstract: DNA duplex separation and formation underlie our most fundamental genetic processes. One of the most powerful tools for exploring these binding and unbinding reactions is single-molecule force spectroscopy. This method applies tension to a single DNA strand and observes the change in its extension or kinetics with a microscope. However, these experiments typically study longer DNA molecules (>10 bp) subject to higher forces (> 10 bp); hence, the behavior of short DNA subject to weak forces is not well understood. In particular, it is not clear whether statistical chain models ordinarily used to explain force spectroscopy data are applicable at these small scales. To remedy this, we focused solely on short DNA duplexes (